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The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Natural Products and Bioprospecting

Article Title: Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway

doi: 10.1007/s13659-025-00582-z

Figure Lengend Snippet: The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: ZO-1 , sc-33725 , 1: 200 (IF) , Mouse , Santa Cruz Biotechnology.

Techniques: Immunofluorescence, Staining, Activity Assay, Western Blot